Brand

MultiSciences

Cat Num

70-EK1175

Product Name

Human -fetoprotein/AFP ELISA Kit

Customs Name

Human -fetoprotein/AFP ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened)standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

100 l (prediluted)

Sensitivity

0.45 pg/ml

Standard Curve Range

31.25 - 2000 pg/ml

Spike Recovery Range

90% - 110%

Mean Spike Recovery

0.98

CV of Intra plate

1.6 % - 2.2 %

CV of Inter plate

1.6 % - 3.3 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against AFP
Human AFP Standard, lyophilized
AFP Detect Antibody
Assay Buffer (10)
Substrate (TMB)
Stop Solution
Washing Buffer (20)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human AFP. The Human -fetoprotein/AFP ELISA is for research use only. Not for diagnostic or therapeutic procedures.
-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. AFP is measured in pregnant women through the analysis of maternal blood or amniotic fluid, as a screening test for a subset of developmental abnormalities. AFP will be elevated in some of the diseases, including omphalocele, hepatocellular carcinoma/hepatoma, neural tube defects, nonseminomatous germ cell tumors, yolk sac tumor, ataxia telangiectasia.